pcp [2018-05-28 09:36:58]

pyrrolidone-carboxylate peptidase

locus: BSU02650

pI: 6.42

mw: 23.63 kDa

protein length: 215 aa

gene length: 645 bp

function: removal of the N-terminal pyroglutamyl group from peptides

product: pyrrolidone-carboxylate peptidase

essential: no

ec: 3.4.11.8

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.8|Protein modification/ other]

Gene

Coordinates: 286,773 → 287,420

The protein

Catalyzed reaction/ biological activity

• Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro (according to Swiss-Prot)

Protein family

• peptidase C15 family (according to Swiss-Prot)

Structure

• [PDB|1AUG] (from B. amyloliquefaciens, 72% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|13E80EAC33C30BB0BBF08A0F0E37EB18CC87C8CA|pcp]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE02650 (Δ[gene|13E80EAC33C30BB0BBF08A0F0E37EB18CC87C8CA|pcp]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCCATCTCCTTTTT, downstream forward: _UP4_CACTAAGCGGCCGCCGCTGT
• BKK02650 (Δ[gene|13E80EAC33C30BB0BBF08A0F0E37EB18CC87C8CA|pcp]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCCATCTCCTTTTT, downstream forward: _UP4_CACTAAGCGGCCGCCGCTGT

References

• 1362573,1353026